GLYcoPROFILE used to evaluate sialidases ligands efficiency

Polyvalent transition‐state analogues of sialyl substrates strongly inhibit bacterial sialidases.

C. Assailly, C. Bridot, A. Saumonneau, P. Lottin, B. Roubinet, E-M. Krammer, F. François, F. Vena, L. Landemarre, D. Alvarez Dorta, D. Deniaud, C. Grandjean, C. Tellier, S. Pascual, V. Montembault, L. Fontaine, F. Daligault, J. Bouckaert, S. G. Gouin., A., Chem. Eur. J., 2020, (DOI : 10.1002/chem.202004672).

 

GLYcoPROFILE technology was recently used in bacterial sialidases studies to screen a large range of inhibitors. Trough this method, polyvalent compounds based on the transition state sialyl cation 2-deoxy-2,3-didehydro-N-acetylneuraminic (DANA) have shown high affinity for sialidase from S.pneumoniae (NanA) from micromolar to picomolar range.

 

GLYcoPROFILE technology is an useful tool for characterisation of new ligands. A range of LEctPROFILE kits developed by GLYcoDiag are coming soon for your research needs (i.e. Sialylation (NeuAc, NeuGc, α2,3/α2,6), Galili, DC-SIGN & Langerin, …).

 

Cells GLYcoPROFILE used to evaluate beta-glucan effects on cutaneous cells

Laminarin Effects, a β-(1,3)-Glucan, on Skin Cell Inflammation and Oxidation
Ozanne, H., Toumi, H., Roubinet, B., Landemarre, L., Lespesailles, E., Daniellou, R., Cesaro, A., Cosmetics, 2020, 7, 66.

 

GLYcoPROFILE was recently used to evaluate on cutaneous cells (i.e. NHDFa and NHEK) the effect of Laminarin, a β-(1,3)-glucan from the seaweed Laminaria digitata, polysaccharide which provides anti-inflammatory and anti-oxidative properties.

This study is another example of the powerfull interest to use the GLYcoPROFILE technology to investigate biological properties of glycomolecules.

GLYcoPROFILE® used for the developement of emotional active ingredient

Queiroz, A., Landemarre, L., Vial, F., Aubert, A., IFSCC Magazine 1 2020

The GLYcoPROFILE® study was used recently in the development of a new cosmetic ingredient developped by Seqens. This study highlights the ability of a high molecular weight exopolysaccharide to provide a quantifiable benefit versus placebo by visibly enhancing not only the quality of the skin tissue but also the consumer’s emotional state. In fact, the GLYcoPROFILE have enabled to highlight specific interaction with rhamnose specific lectin (lectin CorM which recognizes rhamnosylated glycan structures in the same manner as rhamnose-specific protein expressed at the surface of keratinocytes and fibroblasts). Apart from the fact that rhamnose glycan motif recognition is known to interfere with cells and epidermal regeneration, thus having anti-aging and anti-inflammatory properties, rhamnose recognition is known to enhance beta-endorphin release by keratinocytes and improve the feeling of wellbeing.

A review about Galactofuranose-related Enzymes

Senecar, M., Lafite, P., Eliseeva, S. V., Petoud, S., Landemarre, L., Daniellou, R., Int. J. Mol. Sci. 2020, 21, 3465; doi:10.3390/ijms21103465

Galactofuranose is a rare form of the well-known galactose sugar, and its occurrence in numerous pathogenic micro-organisms makes the enzymes responsible for its biosynthesis interesting targets. This review summarizes the role of these carbohydrate-related proteins with a special emphasis on the galactofuranosidases recently characterized as an efficient recombinant biocatalyst.