GLYcoDiag: Microbiology services pricing 2024 available

Since it’s creation, GLYcoDiag has proposed several miniaturized methods (Micro-challenge test, Time-to-kill study, Minimal inhibitory concentration (MIC), Co-culture studies and microbiota skin adhesion) to accelerate the development of your cosmetic actives. The 2024 pricing of these standardised methods are now available with a slight but controlled increase due to our supplier’s costs changes. However, we decided this year to maintain the Micro-challenge test price similar to last year. Indeed, in a period of inflation, this was only possible with a strong control of the reagents and consumables costs by our team.

Don’t hesitate to contact us to obtained more informations and/or a quotation about these analyses.

GLYcoDiag services & products intended for the study of glycobiological interactions

Since its creation in 2005, GLYcoDiag has continuously extended its expertise in the field of glycoscience in order to propose you ever more innovative services and products that suits your needs.

Studies:

Use GLYcoPROFILE plateform for:

Identify glycans signature of your therapeutic recombinant glycoproteins for in-process reference sample comparison, batch to batch monitoring, glycoforms specific profiles or immunogens glycans detection.

– Study glycome expression and accessibility for the research of new glyco-biomarkers.

– Search and characterize new glycosylated related drugs or molecule and anticipate potential actionon biological mechanism.

– Study the effect of products on glycobiological interactions between microorganisms and cells (Glycomicrobiote studies).

Glycoanalytics.

Complementary to our GLYcoPROFILE plateform we propose several standard glycoanalysis to know more about the structure of your product: O- and N-glycans, Sialic acid composition, neutral monosaccharide composition, and molecular weight.

Products:

GLYcoDiag develop it’s own range of research reagents and kits dedicated to Glycoscience and add new reference every years in order to offer to our customer the most original, innovative and better quality products:

– Natural and recombinant lectins;

Neoglycoproteins;

LEctPROFILE® affinity chromatography supports;

LEctPROFILE® kits for evaluation of crude or purified glycoconjugates interactions with lectins.

The full list of these reagents are available directly on our website at: https://www.glycodiag.com/products

Don’t hesitate to contact us, if you need: (1) further information about GLYcoDiag’s services and products, (2) a quotation.

Light on antimicrobial services: Micro-challenge test

As you know GLYcoDiag provides it’s micro-challenge test services from more than 10 years. Compared to the standard method, the GLYcoDiag’s micro-challenge test is a high throughput, cost effective and the method of choice for research projects aiming at the development of new system preservatives or the control steps of anti-microbial efficacy where the regulatory method is not absolutely required. Now, we propose two additional upgraded models of our micro-challenge test method:
(1) Re-challenge testing. Repeated inoculation with specific test micro-organisms can show the number of contamination challenges a product can withstand before the preservative system fails for the micro-organism studied.
(2) Mixed. In this study, the goal is to compare and determine the efficiency of a preservative with a mixture of microbial strains (fungi and bacteria), in a more complex environment model.

Next micro-challenge test studies will be launched in week S6 (reception of products before the end of week S5).

 

 

Latest GLYcoDiag’s publications

1. Last publication about the use of our FimH and DC-SIGN LEctPROFILE kits for the design of new gold mannoside nanoparticles. We thank Dr.Stephane Vincent (from the University of namur) to give us the chance to be associated with this wonderful work.

Wang, T. et al. “Glycofullerene-AuNPs as multivalent ligands of DC-SIGN and bacterial lectin FimH: tuning nanoparticule size and ligand density”. Nanoscale, 2023, 15, 11657-11666. DOI: https://doi.org/10.1039/D3NR01611K

2. First publication of the collaborative work (ANR Cyclick) between University of Le Mans (IMMM), University of Nantes (CEISAM) and GLYcoDiag about the synthesis of linear glycopolymers and their evaluations through lectins interactions (ConA, BC2LA, FimH, Langerin and DC-SIGN).

Gonnot, C. et al.ROMP-based Glycopolymers with high Affinity for mannose-binding lectins”. Biomacromolecules, 2023, 14, 3689-3699. https://doi.org/10.1021/acs.biomac.3c00406

GLYcoDiag Light on : Evaluation of antimicrobial activities of cosmetics ingredients

Dear customers,

As you know, GLYcoDiag propose several miniaturized methods in microbiology which help you during the development stages of your ingredients. The methods listed below enable to study the potential antimicrobial properties of a product (alone or in combination) on a large range of bacteria and fungi.

Those methods are devoted to:

– Determine the minimal inhibitory concentration (CMI).

Design boosters, systems preservatives or identification/optimization of synergy effects through micro-challenge test method. As a reminder, micro-challenge test is the method of choice for all steps where the regulatory method is not absolutely required (ISO11930). Moreover, adapted to various products viscosities, the micro-challenge test can help you to accelerate your development through large screening with different formulations. Finally, the micro-challenge test method can be customized according to your needs (strains and picking time).

Evaluate the rate of microbial decrease through Time to kill study.

We remain at your disposal for any further information regarding these services or for a quotation.

With best regards,

GLYcoDiag

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LEctPROFILE® kits – Glycoprofile easily

Dear Madam/Mister,

GLYcoDiag has recently launched new LEctPROFILE® kits (dedicated lectin arrays) designed for efficient evaluation of crude or purified glycoconjugates.

  • LK01FimH (screening of FimH ligands)

  • LK02DC (Research of DC-SIGN and/or Langerin ligands)

  • LK03Gal3 (screening of Galectin-3 inhibitors)

  • LK04Cells (Highlight cells glyco-signatures according to cell-type, differentiation, status (healthy or not) and environment . Evaluation of product effect on glycan expression and cells behaviour. Research of cell glyco-biomarkers)

  • LK05α2,3_α2,6 (Determination of the ratio α2,3/α2,6, study the sialylation levels)

  • LK06Galili (Detection of undesirable Galili antigen on cells, tissues or biotherapeutics)

As a reminder, the LEctPROFILE® kits are convenient for the following relevant applications:

1) Detection of desirable or undesirable glycans during the early stage of quality control of biotherapeutics;

2) Identification of glyco-biomarkers related to cells behaviour and/or pathology;

3) Analysis and characterisation of the glycosylation level of glycoproteins contained in biological fluids (e.g.target the sialylation status of relevant serum biomarkers);

4) Research of ligand for lectins of interest that are well-known to be involved in particular disorder or in biological mechanism of interest.

Visit our website at https://www.glycodiag.com/products/lectprofile-kits/ to download the technical notes of each kit and see several examples of applications.

GLYcoDiag will attend the next 21st Eurocarb symposium in Paris from 09 to 13 July and will be presenting some results obtained with these kits.

Applied glycosciences for cosmetics ingredients development

GLYcoDiag is a testing and research laboratory specialized in Glycosciences and Microbiology. Its own technology platform GLYcoPROFILE® is intended to the study of « glyco-actives » mechanisms relevant for cosmetics applications and the development of new cosmetic ingredients. GLYcoPROFILE® allows to reduce your time development and increase your innovation capability.

The technology is based on the determination of interaction profiles of molecules with glycans binding proteins allowing to identify specific interactions of ingredient containing glycosylated molecules (from raw material to purified molecules), highlight glycans accessibility, identify “glycan signature” and compare with reference GLYcoPROFILEs, anticipate potential biological properties that are relevant for cosmetic applications.

GLYcoPROFILE® platform is declined from analytical/structural studies aiming at the identification of glycan profiles (which also shape with glycan content), accessibility and evaluate the potential to modulate glycobiological interaction mechanisms related to specific cosmetics activities (non exhaustive list):

Innate Immunity : a focus on innate immunity is carried out by the use of human recombinant lectins expressed on the surface of skin cells or in their close environment. For example the lectins DC-SIGN, Langerin or Dectin-1 are used to prove/demonstrate the effect of sample on this biological mechanism.

Anti-ageing : Determination of the interaction profile of ingredient with a panel of human recombinant lectins (e.g. CD44, layilin) ​​recognizing glycoaminoglycan motifs. These receptors are expressed on the surface of skin cells or in their close environment and are involved in cell development, tissue remodelling and inflammation.

Pigmentation : Ingredient effect on pigmentation is evaluated through the study of its interaction with lectins whose specificity correspond to those involved in melanin transfer from melanocytes to keratinocytes.

Well-being : Wellness potential action of an ingredient is predicted by evaluating the potential of interaction of the sample with lectins that recognised specific glycans structures. Indeed, some specific glycans structure bearing oligo- polysaccharides are well-known to be involved in secretion of β-endorphine (an hormone produced during wellness feeling).

From analytics to the potential activity, GLYcoPROFILE® platform and dedicated studies help you to make the difference.