GLYcoDiag is a testing and research laboratory specialized in Glycosciences and Microbiology. Its own technology platform GLYcoPROFILE^® is intended to the study of « glyco-actives » mechanisms relevant for cosmetics applications and the development of new cosmetic ingredients. GLYcoPROFILE^® allows to reduce your time development and increase your innovation capability.

The technology is based on the determination of interaction profiles of molecules with glycans binding proteins allowing to identify specific interactions of ingredient containing glycosylated molecules (from raw material to purified molecules), highlight glycans accessibility, identify “glycan signature” and compare with reference GLYcoPROFILEs, anticipate potential biological properties that are relevant for cosmetic applications.

GLYcoPROFILE^® platform is declined from analytical/structural studies aiming at the identification of glycan profiles (which also shape with glycan content), accessibility and evaluate the potential to modulate glycobiological interaction mechanisms related to specific cosmetics activities (non exhaustive list):

Innate Immunity : a focus on innate immunity is carried out by the use of human recombinant lectins expressed on the surface of skin cells or in their close environment. For example the lectins DC-SIGN, Langerin or Dectin-1 are used to prove/demonstrate the effect of sample on this biological mechanism.

Anti-ageing : Determination of the interaction profile of ingredient with a panel of human recombinant lectins (e.g. CD44, layilin) ​​recognizing glycoaminoglycan motifs. These receptors are expressed on the surface of skin cells or in their close environment and are involved in cell development, tissue remodelling and inflammation.

Pigmentation : Ingredient effect on pigmentation is evaluated through the study of its interaction with lectins whose specificity correspond to those involved in melanin transfer from melanocytes to keratinocytes.

Well-being : Wellness potential action of an ingredient is predicted by evaluating the potential of interaction of the sample with lectins that recognised specific glycans structures. Indeed, some specific glycans structure bearing oligo- polysaccharides are well-known to be involved in secretion of β-endorphine (an hormone produced during wellness feeling).

From analytics to the potential activity, GLYcoPROFILE^® platform and dedicated studies help you to make the difference.

GLYcoDiag will be present at the next Cosm’innov congress the 24-25th May 2023 and will present it’s last innovations concerning microbiota services devoted to cosmetics ingredients.

Download the Poster “Microbiota co-culture models for cosmetics ingredients evaluation: Metabolism and skin adhesion studies”

With more than 15 years of expertise in glycobiological interactions studies and complex carbohydrate analyses, GLYcoDiag continues to help its customers to speed-up their biotherapeutic projects through its GLYcoPROFILE^® platform.

The GLYcoPROFILE^® is commonly used in a large range of applications as follow:

– Drug design/Drug discovery: For the research and evaluation of new glycosylated drugs on a range of lectins (recombinant human or bacterial) known to be involved in biological process (e.g. innate immunity, bacterial interaction,…);

– Development of in-process quality control of therapeutic recombinant glycoproteins: identification of specific glycan signatures of your reference product, batch-to-batch monitoring, glycoform specific profiles and immunogen glycan detection (Galα1,3Gal (Galili antigen), Neu5Gc,…);

– Discovery of relevant glyco-biomarkers: through utilization of specialized kits to highlight glycan signatures (e.g. cell surface glycosylation monitoring).

Download the Flyer “Biotech” that summarizes the full description of the GLYcoPROFILE^® platform used for the biotherapeutics development projects. Since the beginning of this year, several LEctPROFILE^® kits are now available for your glycosciences research needs. Have a look directly on our website at https://www.glycodiag.com/products/lectprofile-kits/ to know more about these kits.

Don’t hesitate to contact us for discussion about your research and development projects in glycosciences.

As you know, GLYcoDiag is specialized in glycosciences services and products for the biotech, pharma, veterinary, cosmetic and diagnostic industries. We provide a large range of glycosciences reagents such as lectins, neoglycoproteins, lectin plates, and affinity gels to speed up your research and development studies. GLYcoDiag now launch six LEctPROFILE® kits (dedicated lectin arrays) designed for efficient evaluation of crude or purified glycoconjugate interactions.

The LEctPROFILE® kits are convenient for the following relevant applications:

1) Detection of desirable or undesirable glycans during the early stage of quality control of biotherapeutics;

2) Identification of glyco-biomarkers related to cells behaviour and/or pathology;

3) Analysis and characterisation of the glycosylation level of glycoproteins contained in biological fluids or to target the sialylation status of relevant serum biomarkers;

4) Research of ligand for lectins of interest that are well-known to be involved in particular disorder or in biological mechanism.

The LEctPROFILE® kits enable a fast measurement (below 3 h) and are easily accessible to all (only a microplate reader is required). Each kit is composed of a 96-well plate containing immobilised target lectin, and supplemented with the corresponding tracer and the revealing solution.

The LEctPROFILE^® kits available are:

• LK01FimH (screening of FimH ligands)

• LK02DC (Research of DC-SIGN/Langerin differential ligands)

• LK03Gal3 (screening of Galectin-3 inhibitors)

• LK04Cells (Highlight cells glyco-signatures according to cell-type, environment and/or pathology. Evaluation of product effect on glycan expression and/or accessibilities. Research of cell glyco-biomarkers)

• LK05α2,3_α2,6 (Determination of the ratio α2,3/α2,6, study the sialylation levels)

• LK06Galili (Detection of undesirable Galili antigen on biotherapeutics)

Visit our website at https://www.glycodiag.com/products/lectprofile-kits/ to download the technical notes of each kit and see several examples of applications.

Do not hesitate to contact us for more information regarding these kits or other GLYcoDiag’s products.

GLYcoDiag is your partner of microbiological studies (mainly in cosmetics field). GLYcoDiag’s expertises are used in the research and optimisation of the new active compounds, prebiotics, combination of compounds (synergy effect), boosters, systems preservatives and in the validation of the new formulations.

GLYcoDiag help you for microbiote studies:

– Co-cultures models: GLYcoDiag has developed co-culture models which allow to study the effect of a cosmetic product on the growth and/or the decrease of each strain growing together in the same medium and environmental conditions. All of the co-culture models developed by GLYcoDiag are based on the following criteria :

– Referenced strains either from well-known collections or isolated and characterized wild-type strains from GLYcoDiag’s own cell bank.

– Calibrated inoculum of each strain mixed (four to five strains) in proportion mimicking eubiosis equilibrium or dysbiosis related to specific cutaneous diseases.

– A common culture medium based on minimum nutrients, allowing to keep the growth of each strain in at wanted proportions during the study (usually 24 to 72h).

– Respective pH and temperature according to the targeted environment.

– Miniaturized, accurate and reliable.

GLYcoDiag help you for alternative microbiology services:

– Micro-challenge test : screening of anti-microbial activities of many samples simultaneously.

– Time Kill study : determination of the kinetic of inhibition of strain growth (bacteria and/or fungi)

– Minimal Inhibition concentration (MIC)/Growth effect : Determination of microbial properties of product on a variety of microorganisms.

– Glycomicrobiote: study the effect of your products on microorganisms interactions (one or up to 4 strains simultaneously) with the skin cells (i.e. Human normal keratinocytes (NHEK), corneocytes, …).

Don’t forget: GLYcoDiag will be present at the next In cosmetics global at Barcelona (28^th-30^th March). Do not hesitate to visit us during this event on booth Y33 to know more about our services.