Description
The LEctPROFILE kit allows efficient evaluation of crude or purified glycoconjugates interactions (i.e. synthetic molecules or glycoconjugates) with lectins by a simple measurement of absorbance or fluorescence. The LectPROFILE kit enables a fast measurement (below 3 h) and are easily accessible to all.
Each kit are composed of a 96-well plate immobilised with the target lectin, the corresponding tracor and the revealing solution.
All our lectins are controlled under a standardized procedure assessed by interactions with specific neoglycoproteins or glycoproteins through GLYcoPROFILE method.
Applications
The evaluation of compounds interactions with lectins is possible by two different modes :
- Direct Binding to evaluate potential interaction of compounds ranging from pure molecule to complex mixtures ( glycocojugates(s), complex carbohydrates pr glycomometics). Previous labeling of target molecule(s) by biotinylation or by fluoresceinylation is required for readout.
- Competition Mode : Competitive interaction between a sample and a specific labelled tracer know to have good affinity for the lectin without preliminary labelling of sample. This mode is typically used for the evaluation of the IC50- value (concentration corresponding to 50% of lection inhibition), for the screening of potential candidates, for avidity comparison or for batch to batch monitoring.
| NAME | SPECIFICITY | KIT CONTENT | ANALYSIS MODE | STABILITY |
| FimH LEctPROFILE kit | High mannosylated structure glycan(s) | 1 x 96 microplate well for fluorescence detection; Assay reagents: FimH tractor & Streptavidine-DTAF solutions | Competition Mode: until 5-8 samples analysed in triplicate.
Direct Binding: until 10 samples analysed in triplicate at 3 concentrations |
6 months at -20°C |
| LEctPROFILE kit | -a | 1 x 96 microplate well for fluorescence or absorbance detection; Assay reagents: lectin tracor & revealing solution | Competition Mode: until 5-8 samples analysed in triplicate
Direct Binding: until 10 samples analysed in triplicate at 3 concentrations |
-b |
Table1. Specifications of LEctPROFILE kits. a. For specificity of LEctPROFILE kits, see our lectins specificities table. b. Each LEctPROFILE kit are stable for minimum 6 months at -20°C.
