LEctPROFILE Kits

Description

The LEctPROFILE kit allows efficient evaluation of crude or purified glycoconjugates interactions (i.e. synthetic molecules or glycoconjugates) with lectins by a simple measurement of absorbance or fluorescence. The LectPROFILE kit enables a fast measurement (below 3 h) and are easily accessible to all.

Each kit are composed of a 96-well plate immobilised with the target lectin, the corresponding tracor and the revealing solution.

All our lectins are controlled under a standardized procedure assessed by interactions with specific neoglycoproteins or glycoproteins through GLYcoPROFILE method.

Applications

The evaluation of compounds interactions with lectins is possible by two different modes :

- Direct Binding to evaluate potential interaction of compounds ranging from pure molecule to complex mixtures ( glycocojugates(s), complex carbohydrates pr glycomometics). Previous labeling of target molecule(s) by biotinylation or by fluoresceinylation is required for readout.

- Competition Mode : Competitive interaction between a sample and a specific labelled tracer know to have good affinity for the lectin without preliminary labelling of sample. This mode is typically used for the evaluation of the IC50- value (concentration corresponding to 50% of lection inhibition), for the screening of potential candidates, for avidity comparison or for batch to batch monitoring.

NAME SPECIFICITY KIT CONTENT ANALYSIS MODE STABILITY
FimH LEctPROFILE kit High mannosylated structure glycan(s) 1 x 96 microplate well for fluorescence detection; Assay reagents: FimH tractor & Streptavidine-DTAF solutions Competition Mode: until 5-8 samples analysed in triplicate.

Direct Binding: until 10 samples analysed in triplicate at 3 concentrations

6 months at -20°C
LEctPROFILE kit -a 1 x 96 microplate well for fluorescence or absorbance detection; Assay reagents: lectin tracor & revealing solution Competition Mode: until 5-8 samples analysed in triplicate

Direct Binding: until 10 samples analysed in triplicate at 3 concentrations

-b

Table1. Specifications of LEctPROFILE kits. a. For specificity of LEctPROFILE kits, see our lectins specificities table. b. Each LEctPROFILE kit are stable for minimum 6 months at -20°C.