Neoglycoproteins
Neoglycoproteins are glycosylated bovine serum albumin (BSA) molecules obtained either after the conjugation of a phenylisothiocyanate glycosides with the ε-amino groups of lysine residues of BSA or by Click Chemistry between an alcyne (or azoture) glycoside with a BSA-azoture (or BSAalcyne). The synthesis of each neoglycoprotein is conducted under a standardized procedure allowing excellent batch to batch reliability. Each neoglycoprotein is submitted to a complete quality control ensuring a total conformity with the specifications : purity, carbohydrates/protein ratio, labeling and functionnality assessed by interactions with lectins through GLYcoPROFILE.
Mono and di-saccharide neoglycoproteins are produced routinely and always available (from 1 mg to 50 mg) in unlabeled or fluoresceinylated forms (Biotin, Fluorescein, Cyanine 5.5). Customized neoglycoproteins with others glycoconjugates as well as others carriers are available upon request.
Intended use & Benefits:
Neoglycoproteins are known as “amplifiers” of carbohydrates-proteins interactions. The use of neoglycoproteins as tools to decipher glycoconjugates, carbohydrates binding proteins and more generally proteins-carbohydrates interactions were described in many studies. Indeed, neoglycoproteins are used in : histochemistry, ELISA assays, blotting assays, affinity chromatography, ITC, SPR, flow cytometry, confocal or electron microscopy.
Neoglycoproteins can be used for research purposes to:
- Identify lectins or lectin-like proteins.
- Purify lectins or other carbohydrate-binding proteins.
- Design new diagnostic tools.
- Discover biomarkers.
- Target drugs.
- Trigger immune response against carbohydrates moieties.
The avidity of the neoglycoproteins is 102 -104 higher than corresponding free sugars.
The neoglycoproteins are very reliable and stable products that can be labeled with great flexibility.
The high solubility in aqueous solutions makes neoglycoproteins very powerfull reagents for glycosciences studies.
Detailed product list:
Search into GLYcodiag's database the availability of your product :
Reference | Description | Glycan structure | Coupling ratio | |
---|---|---|---|---|
NeoCT | βChitobiose-BSA | βChitobiose | Between 10 to 20 | |
NeoF | αLFuc-BSA | αLFucose | Between 10 to 20 | |
NeoGa | αDGal-BSA | αDGalactose | Between 10 to 20 | |
NeoGaP | βDGal6P-BSA | βDGalactose-6-phosphate | Between 10 to 20 | |
NeoGaN | αDGalNAc-BSA | αD-N-acetylgalactosamine | Between 10 to 20 | |
NeoGaf | αGalactofuranose-BSA | αGalactofuranose | Between 10 to 20 | |
NeoaG | αDGlc-BSA | αDGlucose | Between 10 to 20 | |
NeobG | βGlc-BSA | βGlucose | Between 10 to 20 | |
NeoGN | βDGlcNAc-BSA | βD-N-acetylglucosamine | Between 10 to 20 | |
NeoGU | βDGlucuronic acid-BSA | βDGlucuronic acid | Between 10 to 20 | |
NeoGol | Glucitol-BSA-Fv | Glucitol | Between 10 to 20 | |
NeoL | βDLac-BSA | βDLactose | Between 10 to 20 | |
NeoM | αDMan-BSA | αDManose | Between 10 to 20 | |
NeoMP | αDMan6P-BSA | αDManose-6-phosphate | Between 10 to 20 | |
NeoR | αLRhamnose-BSA | αLRhamnose | Between 10 to 20 | |
NeoX | βDXylose-BSA | βDXylose | Between 10 to 20 | |
NeoGaliliL | Galα1,3Gal-BSA | Galili : Galα1,3Gal | Between 5 to 10 | |
NeoNeuAcL | αNeuAc-BSA | N-acetylneuraminic acid (Neu5Ac) | Between 5 to 10 | |
NeoNeuGcL | αNeuGc-BSA | N-glycolylneuraminic acid (Neu5Gc) | Between 5 to 10 | |
NeoLex | Galβ1-4(Fucα1-3)GlcNAcβ1- 3GalNAc-BSA | Lewis X antigen : Galβ1-4(Fucα1- 3)GlcNAcβ1-3GalNAc | Between 5 to 10 | |
NeoSLex | Neu5Acα2-3-Galβ1-4(Fucα1- 3)GlcNAcβ1-3GalNAc-BSA | Sialyl Lewis X antigen : Neu5Acα2-3-Galβ1- 4(Fucα1-3)GlcNAcβ1-3GalNAc | Between 5 to 10 | |
NeoSTn | Neu5Acα6GalNAc-BSA | STn-antigen : Neu5Acα6GalNAc | Between 5 to 10 | |
NeoT | Galβ3GalNAc-BSA | T-antigen : Galβ3GalNAc | Between 10 to 15 | |
NeoTn (=NeoGaN) | αDGalNAc-BSA | Tn-antigen : αD-N-acetylgalactosamine | Between 10 to 20 | |
Neo3’SL | 3’Sialylactose-BSA | 3’Sialylactose | Between 3 to 5 | |
Neo6’SL | 6’Sialylactose-BSA | 6’Sialylactose | Between 3 to 5 |