Neoglycoproteins

Neoglycoproteins are glycosylated bovine serum albumin (BSA) molecules obtained either after the conjugation of a phenylisothiocyanate glycosides with the ε-amino groups of lysine residues of BSA or by Click Chemistry between an alcyne (or azoture) glycoside with a BSA-azoture (or BSAalcyne). The synthesis of each neoglycoprotein is conducted under a standardized procedure allowing excellent batch to batch reliability. Each neoglycoprotein is submitted to a complete quality control ensuring a total conformity with the specifications : purity, carbohydrates/protein ratio, labeling and functionnality assessed by interactions with lectins through GLYcoPROFILE.

Mono and di-saccharide neoglycoproteins are produced routinely and always available (from 1 mg to 50 mg) in unlabeled or fluoresceinylated forms (Biotin, Fluorescein, Cyanine 5.5). Customized neoglycoproteins with others glycoconjugates as well as others carriers are available upon request.

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Intended use & Benefits:

Neoglycoproteins are known as “amplifiers” of carbohydrates-proteins interactions. The use of neoglycoproteins as tools to decipher glycoconjugates, carbohydrates binding proteins and more generally proteins-carbohydrates interactions were described in many studies. Indeed, neoglycoproteins are used in : histochemistry, ELISA assays, blotting assays, affinity chromatography, ITC, SPR, flow cytometry, confocal or electron microscopy.

Neoglycoproteins can be used for research purposes to:

  • Identify lectins or lectin-like proteins.
  • Purify lectins or other carbohydrate-binding proteins.
  • Design new diagnostic tools.
  • Discover biomarkers.
  • Target drugs.
  • Trigger immune response against carbohydrates moieties.

The avidity of the neoglycoproteins is 102 -104 higher than corresponding free sugars.
The neoglycoproteins are very reliable and stable products that can be labeled with great flexibility.
The high solubility in aqueous solutions makes neoglycoproteins very powerfull reagents for glycosciences studies.

Detailed product list:

ReferenceDescriptionGlycan structureCoupling ratio
NeoCTβChitobiose-BSAβChitobioseBetween 10 to 20
NeoFαLFuc-BSAαLFucoseBetween 10 to 20
NeoGaαDGal-BSAαDGalactoseBetween 10 to 20
NeoGaPβDGal6P-BSAβDGalactose-6-phosphateBetween 10 to 20
NeoGaNαDGalNAc-BSAαD-N-acetylgalactosamineBetween 10 to 20
NeoGafαGalactofuranose-BSAαGalactofuranoseBetween 10 to 20
NeoaGαDGlc-BSAαDGlucoseBetween 10 to 20
NeobGβGlc-BSAβGlucoseBetween 10 to 20
NeoGNβDGlcNAc-BSAβD-N-acetylglucosamineBetween 10 to 20
NeoGUβDGlucuronic acid-BSAβDGlucuronic acidBetween 10 to 20
NeoGolGlucitol-BSA-FvGlucitolBetween 10 to 20
NeoLβDLac-BSAβDLactoseBetween 10 to 20
NeoMαDMan-BSAαDManoseBetween 10 to 20
NeoMPαDMan6P-BSAαDManose-6-phosphateBetween 10 to 20
NeoRαLRhamnose-BSAαLRhamnoseBetween 10 to 20
NeoXβDXylose-BSAβDXyloseBetween 10 to 20
NeoGaliliLGalα1,3Gal-BSAGalili : Galα1,3GalBetween 5 to 10
NeoNeuAcLαNeuAc-BSAN-acetylneuraminic acid (Neu5Ac)Between 5 to 10
NeoNeuGcLαNeuGc-BSAN-glycolylneuraminic acid (Neu5Gc)Between 5 to 10
NeoLexGalβ1-4(Fucα1-3)GlcNAcβ1- 3GalNAc-BSALewis X antigen : Galβ1-4(Fucα1- 3)GlcNAcβ1-3GalNAcBetween 5 to 10
NeoSLexNeu5Acα2-3-Galβ1-4(Fucα1- 3)GlcNAcβ1-3GalNAc-BSASialyl Lewis X antigen : Neu5Acα2-3-Galβ1- 4(Fucα1-3)GlcNAcβ1-3GalNAcBetween 5 to 10
NeoSTnNeu5Acα6GalNAc-BSASTn-antigen : Neu5Acα6GalNAcBetween 5 to 10
NeoTGalβ3GalNAc-BSAT-antigen : Galβ3GalNAcBetween 10 to 15
NeoTn (=NeoGaN)αDGalNAc-BSATn-antigen : αD-N-acetylgalactosamineBetween 10 to 20
Neo3’SL3’Sialylactose-BSA3’SialylactoseBetween 3 to 5
Neo6’SL6’Sialylactose-BSA6’SialylactoseBetween 3 to 5