CarbPROFILE gels

CarbPROFILE gels are monosaccharides-Sepharose affinity matrices used for purification of specific carbohydrate-binding proteins. The carbohydrates are attached through their non reducing hydroxyl group after pre-activation of sepharose matrix by divinylsulfone (DVS) (see scheme 1 below). The binding of lectins and carbohydrates binding proteins to carbohydrate affinity gel is non-covalent and reversible with high capacity. Lectins and carbohydrates binding proteins are both usually stable compounds which can be recovered by competitive elution (i.e. 0.2 to 0.5 M of monosaccharide) or by modulations of pH and/or ionic strength in high yield and purity.

Applications

Purification of large range of glycoproteins which recognize specific carbohydrates moeities.

Preparation and quality control

Home made carbohydrate affinity chromatography media (CarbPROFILE) prepared in our laboratory.

• Useful for purification of specific carbohydrate-binding proteins (i.e. lectins, lectins-like, GAG-binding proteins from naturals or recombinant sources).
• High yield and purity under mild conditions (i.e. by competitive elution with corresponding monosaccharide or by modulation of pH and/or inonic strength).
• Each CarbPROFILE gel is carefully controled according to our specifications:
• Gel capacity (binding of validated standard over 15 mg/mL of gel)
• Elution yield (over 85 % of injected standard recovered)
• CarbPROFILE gel are available in 5, 10 and 25 mL slurry. Higher quantity are available upon request.