LEctPROFILE® kits
The LEctPROFILE® kits allow efficient evaluation of crude or purified glycoconjugate interactions (i.e. synthetic molecules or glycoconjugates) with lectins by a simple measurement of absorbance or fluorescence. The LEctPROFILE® kits enable a fast measurement (below 3 h) and are easily accessible to all. Each kit is composed of a 96-well plate containing immobilised target lectin, and supplemented with the corresponding tracer and the revealing solution. All our lectins are control quality proofed by a standardized procedure assessed by interactions with specific neoglycoproteins or glycoproteins through GLYcoPROFILE® method.
Applications
LEctPROFILE® kits are designed for relevant applications such as:
- Detection of desirable or undesirable glycans during the early stage of quality control of biotherapeutics;
- Identification of glyco-biomarkers related to cells behaviour and/or pathology;
- Analysis and characterisation of the glycosylation level of glycoproteins contained in biological fluids or to target the sialylation status of relevant serum biomarkers;
- Research of antagonist for lectins of interest that are well-known to be involved in particular disorder or in biological mechanism.
How to use LEctPROFILE® kit?
The evaluation of compound interactions with lectins on LEctPROFILE® kits are possible by two different modes:
Competition Mode : Competitive interaction between a sample and a specific labelled tracer known to have a good affinity for the lectin without preliminary labeling of sample. This mode is typically used for the evaluation of the IC50-value (i.e. concentration corresponding to 50% of lectin inhibition), for the screening of potential candidate, for avidity comparison or for batch to batch monitoring.
Direct Binding is used to evaluate the potential interaction of compounds ranging from pure molecule to complex mixtures (glycocojugate(s), complex carbohydrates or glycomimetics). Previous labeling of target molecule(s) by biotinylation or by fluoresceinylation is required for readout.
Additionally, and under request, the customization and development of a LEctPROFILE® kit according to your needs is possible. Indeed, we have access to a large choice of lectin from natural or recombinant sources (bacterial and human) that have been already validated in our GLYcoPROFILE® plateform.
Stability
Reagents included in the kit are stable for 6 months at +4°C. LEctPROFILE plate is stable for 6 months at – 20°C.
Intended use
The LEctPROFILE kits are for research use only
Detailed product list:
Search into GLYcodiag's database the availability of your product :
| Reference | Name | Specificity | Kit Content | Analysis-mode | |
|---|---|---|---|---|---|
| LK07 | ASGPR LEctPROFILE® kit | GalNAc glycans structures |
| Direct Binding: Up to 10 samples analysed in triplicate at 3 concentrations Competition Mode: Up to 6 to 8 samples analysed in duplicate | |
| LK04E LK04G LK04M | Cells LEctPROFILE® kit (E : eucaryotic cells, G : germinal cells and M : Microbial cells) | Sialic acid (α2,3/α2,6) glycans structures |
| Direct Binding: Up to 7 samples analysed in triplicate at 2 concentrations Competition Mode: Up to 3 samples analysed in duplicate | |
| LK02 | DC-SIGN & Langerin LEctPROFILE® kit | Mannosylated glycans structures |
| Direct Binding: Up to 7 samples analysed in triplicate at 2 concentrations Competition Mode: Up to 3 samples analysed in duplicate | |
| LK01 | FimH LEctPROFILE® kit | Mannosylated glycans structures |
| Direct Binding: Up to 10 samples analysed in triplicate at 3 concentrations Competition Mode: Up to 6 to 8 samples analysed in duplicate | |
| LK03 | Galectin 3 LEctPROFILE® kit | β-Galactoside glycans structures |
| Direct Binding: Up to 10 samples analysed in triplicate at 3 concentrations Competition Mode: Up to 6 to 8 samples analysed in duplicate | |
| LK06 | Galili LEctPROFILE® kit | Galα1,3Gal glycans structures |
| Direct Binding: Up to 10 samples analysed in triplicate at 3 concentrations Competition Mode: Up to 6 to 8 samples analysed in duplicate | |
| LK08 | Glycoprotein LEctPROFILE® kit | Man, GalNAc, Galβ/α, GlcNAc, Fuc, Sialic acid, Rha, Xyl |
| 3 glycoproteins at 1 concentration or 1 glycoprotein at 3 concentrations in triplicate on 24 lectins | |
| LK05 | α2,3_α2,6 LEctPROFILE® kit | Man, GalNAc, Galβ/α, GlcNAc, Fuc, Sialic acid, Rha |
| 3 differents cells types analysed in triplicate on 22 lectins |