Microbiote specialty

Skin microbiote and GLYcoPROFILE®

pre-clinical studies: Put innovation in your cosmetics ingredients.

GLYcoDiag, with its two expertises in microbiote and glycosciences always aims to accelerate the development of cosmetic and nutraceutic ingredients through innovative and miniaturized methods. Hence, GLYcoDiag offers services in microbiology in order to help its customers in the research and optimisation of new ingredients, prebiotics, associations of compounds (synergy effect), boosters, systems preservatives strategies, or the validation of formulations with taking into account them effects on microbiote.

Last developments applied to skin microbiote:

Cosmetic ingredients activities on skin microbiote can be demonstratedon specific co-culture models mimicking skin environments in order to know the ability of your product to modulate: (1)diversity and growth of microbiote, (2) adhesions of respective microbial strains on corneocytes, (3) protect microbiote from environmental stresses.

Each co-culture model is composed of 4 to 5 strains of bacteria and/or yeasts most commonly found in each skin environment, growing together in the same culture medium and conditions.

Initially, GLYcoDiag offers co-culture models composed of laboratory referenced microorganisms representative of the following environments:

  • Most common skin strains
  • Dry
  • Moist
  • Sebaceous

More recently, GLYcoDiag creates its own wild-type microorganisms skin strains bank from healthy volunteers. Indeed, contrary to the referenced laboratory strains commonly used, these wild-type skin strains are more relevant as they keep natural behaviour according to skin environment were they come from.

First in vitro wild-type bacteria co-culture intended to analyse cosmetic ingredients, that respect the skin microbiota:

Face genuine microbiota testing model

GLYcoDiag has designed a model composed of 5 bacterial strains (C. acnes, C. xerosis, M. luteus, S. epidermidis and S. mitis) isolated from cheeks of healthy individuals. This new model, which mimics the skin environment (temperature, pH, proportions of strains), allows you to study the impact of your cosmetic product and/ or ingredients.

Reliable and accurate, results obtained allow to determine a score which is indicative of the action of cosmetic ingredient: either bactericidal, prebiotic or if it maintains the bacterial population and equilibrium (“microbiome friendly”).

Score evaluation of common cosmetic excipients on the consortium co-culture. The grey histogram represents the maximum score (maximum logarithmic reduction (LRs)).

Score evaluation of preservatives on the consortium co-culture. The grey histogram represents the maximum score (maximum ogarithmic reduction (LRs)).

Score evaluation of neutral molecules on the consortium co-culture. The grey histogram represents the maximum score (maximum ogarithmic reduction (LRs)).

Coming soon:

Skin hair models

Recent studies suggest that the appearance of scalp dandruff is no longer linked exclusively to the colonisation of fungal species, but more generally to an imbalance in the scalp microbiote (especially with bacterial strains S.epidermidis and C.acnes). GLYcoDiag develops two different co-culture models representatives of scalp microbiote.

Contact us for more information.

Cutibacterium acnes phylotypes model

This model targets the two main phylotypes of C. acnes commonly found on the skin (IA1 and II), whose imbalance is linked to the appearance of acne.

Contact us for more information.

Explore Microbiote Expertise
Leverage our advanced microbiology and glycobiology services to enhance your product's performance.