Category Archives: News about GLYcoDiag

A review about Galactofuranose-related Enzymes

Senecar, M., Lafite, P., Eliseeva, S. V., Petoud, S., Landemarre, L., Daniellou, R., Int. J. Mol. Sci. 2020, 21, 3465; doi:10.3390/ijms21103465

Galactofuranose is a rare form of the well-known galactose sugar, and its occurrence in numerous pathogenic micro-organisms makes the enzymes responsible for its biosynthesis interesting targets. This review summarizes the role of these carbohydrate-related proteins with a special emphasis on the galactofuranosidases recently characterized as an efficient recombinant biocatalyst.

FimH LEctPROFILE kit enables to screen and study new FimH ligands based on cellulose derivatives.

Cauwel, M.; Sivignon, A.; Bridot, C.; Nongbe, M. C.; Deniaud, D.; Roubinet, B.; Landemarre, L.; Felpin, F.-X.; Bouckaert, J.; Barnich N.; Gouin, S. G.; Chem. Commun., 2019, 55, 10158-10161 .

The development of a chemical method to covalently functionalize cellulose nanofibers and cellulose paper with mannoside ligands displaying a strong affinity for the FimH adhesin from pathogenic E. coli strains was studied. Mannose-grafted cellulose proved efficient to selectively bind FimH lectin and discriminate pathogenic E. coli strains from non-pathogenic ones. FimH LEctPROFILE plates was used in this study to evaluate the effect of the mannose-grafted cellulose compound over FimH lectin.

Neoglycoclusters

Neoglycoproteins are glycosylated bovine serum albumin (BSA) molecules. In order to improve accessibility and avidity of a carbohydrate-binding proteins, a new version of neoglycoproteins containing spacer arm (i.e. an alkyl spacer or a polyethylene glycol (PEG) chain), were developped and proposed either with monosaccharides/disaccharides or with glycoclusters.

Musa acuminata (BanLec) lectin an original Mannose binding lectin

Musa acuminata lectin (BanLec) is isolated from banana pulp and purified by affinity column chromatography on CarbPROFILE Mannose (see below the focus on GLYcoDiag affinity gel). The  carbohydrate recognition domain of BanLec is mainly specific for mannose. BanLec is available in 2 to 5 mg unlabelled or labelled with biotin or fluorescein.

GLYcoDiag lectin BanLec v1.1