Description
The LEctPROFILE® kit allows efficient evaluation of crude or purified glycoconjugates interactions (i.e. synthetic molecules or glycoconjugates) with lectins by a simple measurement of absorbance or fluorescence. The LEctPROFILE® kit enables a fast measurement (below 3 h) and are easily accessible to all.
Each kit is composed of a 96-well plate immobilised with the target lectin, and supplemented with the corresponding tracor and the revealing solution.
All our lectins are controlled under a standardized procedure assessed by interactions with specific neoglycoproteins or glycoproteins through GLYcoPROFILE® method.
LEctPROFILE® kits are for research use only.
Applications
LEctPROFILE® kits are designed for relevant applications such as:
1) Detection of desirable or undesirable glycans during the early stage of quality control of biotherapeutics;
2) Identification of glyco-biomarkers related to cells behaviour and/or pathology;
3) Analysis and characterisation of the glycosylation level of glycoproteins contained in biological fluids or to target the sialylation status of relevant serum biomarkers;
4) Research of antagonist for lectins of interest that are well-known to be involved in particular disorder or in biological mechanism.
How to use LEctPROFILE® kits
The evaluation of compounds interactions with lectins is possible by two different modes :
- Direct Binding to evaluate potential interaction of compounds ranging from pure molecule to complex mixtures ( glycocojugates(s), complex carbohydrates pr glycomometics). Previous labeling of target molecule(s) by biotinylation or by fluoresceinylation is required for readout.
- Competition Mode : Competitive interaction between a sample and a specific labelled tracer know to have good affinity for the lectin without preliminary labelling of sample. This mode is typically used for the evaluation of the IC50- value (concentration corresponding to 50% of lection inhibition), for the screening of potential candidates, for avidity comparison or for batch to batch monitoring.
| Reference | NAME | SPECIFICITY | KIT CONTENT | ANALYSIS MODE |
| LK01 | FimH LEctPROFILE® kit | High mannosylated glycans structures | - 1 x 96 microplate well for fluorescence detection;
- FimH tracer solution - Streptavidine-DTAF solution |
Competition Mode: up to 6 to 8 samples analysed in duplicate.
Direct Binding: up tp 10 samples analysed in triplicate at 3 concentrations |
| LK02 | DC-SIGN & Langerin LEctPROFILE® kit | Mannosylated glycans structures | - 1 x 96 microplate well for fluorescence detection;
- DC-SIGN/Langerin tracer solution - Streptavidine-DTAF solution |
Competition Mode: up to 3 samples analysed in duplicate.
Direct Binding: up tp 7 samples analysed in triplicate at 2 concentrations |
| LK03 | Galectin-3 LEctPROFILE® kit | β-galactoside glycans structures | - 1 x 96 microplate well for absorbance detection;
- Galectin-3 tracer solution - Streptavidine-Peroxydase solution - OPD Tablets |
Competition Mode: up to 6 to 8 samples analysed in duplicate.
Direct Binding: up tp 10 samples analysed in triplicate at 3 concentrations |
| LK04E
LK04G LK04M |
Cells LEctPROFILE® kit | Man, GalNAc, Galβ/α, GlcNAc, Fuc, Sialic acid, Rha | - 3 x 96 microplate for fluorescence detection
- CFSE labelling solution |
3 differents cells types analysed in each kit |
| LK05 | α2.3_α2.6 LEctPROFILE® kit | Sialic acid (α2.3/α2.6) glycans structures | - 1 x 96 microplate well for fluorescence detection;
- α2.3 tracer solution - α2.6 tracer solution - Streptavidine-DTAF solution |
Competition Mode: up to 3 samples analysed in duplicate.
Direct Binding: up tp 7 samples analysed in triplicate at 2 concentrations |
| LK06 | Galili LEctPROFILE® kit | Galα1.3Gal glycans structures | - 1 x 96 microplate well for absorbance detection;
- Galili tracer solution - Streptavidine-Peroxydase solution - OPD Tablets |
Competition Mode: up to 6 to 8 samples analysed in duplicate.
Direct Binding: up tp 10 samples analysed in triplicate at 3 concentrations |
| LK07 | ASGPr LEctPROFILE® kit | GalNAc glycans structures | '- 1 x 96 microplate well for fluorescence detection – ASGPr tracer solution - Streptavidine-DTAF solution | Direct Binding: Up to 10 samples analysed in triplicate at 3 concentrations Competition Mode: Up to 6 to 8 samples analysed in duplicate |
| LK08 | Glycoprotein LEctPROFILE® kit | Man, GalNAc, Galβ/α, GlcNAc, Fuc, Sialic acid, Rha, Xyl | '- 3 x 96 microplate well for fluorescence detection - Biotine for glycoprotein labelling – Streptavidine-DTAF solution | 3 differents Glycoprotein types analysed in each kit at 1 concentration or 1 glycoprotein at 3 concentrations |
Table1. Specifications of LEctPROFILE kits. a. For specificity of LEctPROFILE kits, see our lectins specificities table. b. Each LEctPROFILE kit are stable for minimum 6 months at -20°C.
Additionally, and under request, the customization and development of a LEctPROFILE® kit according to your needs is possible. Indeed, we have access to a large choice of lectin from natural or recombinant sources (bacterial and human) that have been already validated in our GLYcoPROFILE® plateform.
