Neoglycoproteins:
Neoglycoproteins are glycosylated bovine serum albumin (BSA) molecules obtained after the conjugation of a phenylisothiocyanate glycosides (monosaccharides or disaccharides) with the ε-amino groups of lysine residues of BSA. The synthesis of each neoglycoprotein is conducted under a standardized procedure allowing an excellent batch to batch reliability. Each neoglycoprotein is submitted to a complete quality control ensuring a total conformity with the specifications : purity, carbohydrates/protein ratio, labeling and functionnality assessed by interactions with lectins through GLYcoPROFILE method.
Mono and di-saccharide neoglycoproteins are produced routinely and always available (from 1 mg to 50 mg) in unlabeled or fluoresceinylated forms. Biotinylated or other conjugates as well as more complex neoglycoproteins are available upon request.
Neoglycoclusters:
Our first range of simple neoglycoproteins was achieved by the conjugation of phenylisothiocyanate glycosides with the ε-amino groups of lysine residues of BSA.
In order to improve accessibility and avidity of a carbohydrate-binding proteins, a new version of neoglycoproteins containing spacer arm (i.e. an alkyl spacer), were developed and proposed either with monosaccharides or with glycoclusters.
- Monosaccharide spacer neoglycoproteins:
- Neoglycoclusters, achieved by introduction of a carbohydrate cluster containing 3 to 9 carbohydrates units:
Tumor associated carbohydrate antigens neoglycoproteins :
- T and STn neoglycoproteins with respectively Galβ(1-3)GalNAc and Neu5Ac(a2-6)GalNAc were developed.
- LeX and SLeX neoglycoproteins with respectively Galβ1-4(Fucα1-3)GlcNAcβ1-3GalNA and Neu5Acα2-3-Galβ1-4(Fucα1-3)GlcNAcβ1-3GalNAc were developed
These neoglycoproteins are potentially useful for the research and development of some cancer diagnostics and immunotherapies.
The synthesis of each neoglycocluster is conducted under a standardized procedure allowing an excellent batch to batch reliability. Each neoglycoprotein and neoglycocluster is submitted to a complete quality control ensuring a total conformity with the specifications: purity, carbohydrates/protein ratio, labeling and functionnality assessed by interactions with lectins through GLYcoPROFILE method.
Monosaccharide spacer neoglycoproteins and neoglycocluster are produced routinely and always available (i.e. 1 mg) in unlabeled forms (labeled products available on request).
Intended use:
Neoglycoproteins and Neoglycoclusters are known as “amplifiers” of carbohydrates-proteins interactions. The use of neoglycoproteins as tools to decipher glycoconjugates, carbohydrates binding proteins and more generally proteins-carbohydrates interactions were described in many studies. Neoglycoproteins and Neoglycoclusters are used in number of methods including histochemistry, ELISA assays, blotting assays, affinity chromatography, cytochemistry by flow cytometry, confocal or electron microscopy.
Neoglycoproteins can be use for research purposes to:
- Identify lectins or lectin-like proteins.
- Purify lectins or other carbohydrate-binding proteins.
- Design new diagnostic tools.
- Discover biomarkers.
- Target drugs.
- Trigger immune response against carbohydrates moieties.
Benefits:
- The affinity of the neoglycoproteins is 102-104 higher than that of the corresponding free sugars.
- The neoglycoproteins are very reliable and stable products that can be labeled with great flexibility.
- The high solubility in aqueous solutions makes neoglycoproteins very powerfull reagents for glycosciences studies.
