Neoglycoproteins

Neoglycoproteins are glycosylated bovine serum albumin (BSA) molecules obtained after the conjugation of a phenylisothiocyanate glycosides (monosaccharides or disaccharides) with the ε-amino groups of lysine residues of BSA. The synthesis of each neoglycoprotein is conducted under a standardized procedure allowing an excellent batch to batch reliability. Each neoglycoprotein is submitted to a complete quality control ensuring a total conformity with the specifications : purity, carbohydrates/protein ratio, labeling and functionnality assessed by interactions with lectins.
Mono and di-saccharide neoglycoproteins are produced routinely and always available (from 0.5 mg to 50 mg) in unlabeled or fluoresceinylated forms. Biotinylated or other conjugates as well as more complex neoglycoproteins are available upon request.

Intended use:

Neoglycoproteins are known as “amplifiers” of carbohydrates-proteins interactions. The use of neoglycoproteins as tools to decipher glycoconjugates, carbohydrates binding proteins and more generally proteins-carbohydrates interactions were described in many studies (see bibliography). Neoglycoproteins are used in number of methods including histochemistry, ELISA assays, blotting assays, affinity chromatography, cytochemistry by flow cytometry, confocal or electron microscopy.
Neoglycoproteins can be use for research purposes to:
- Identify lectins or lectin-like proteins.
- Purify lectins or other carbohydrate-binding proteins.
- Design new diagnostic tools.
- Discover biomarkers.
- Target drugs.
- Trigger immune response against carbohydrates moieties.

NGPsimple181126
Neosimples181126

Benefits:

- The affinity of the neoglycoproteins is 102-104 higher than that of the corresponding free sugars.
- The neoglycoproteins are very reliable and stable products that can be labeled with great flexibility.
- The high solubility in aqueous solutions makes neoglycoproteins very powerfull reagents for glycosciences studies.