Author Archives: admin-glycodiag

FimH LEctPROFILE kit enables to screen and study new FimH ligands based on cellulose derivatives.

Cauwel, M.; Sivignon, A.; Bridot, C.; Nongbe, M. C.; Deniaud, D.; Roubinet, B.; Landemarre, L.; Felpin, F.-X.; Bouckaert, J.; Barnich N.; Gouin, S. G.; Chem. Commun., 2019, 55, 10158-10161 .

The development of a chemical method to covalently functionalize cellulose nanofibers and cellulose paper with mannoside ligands displaying a strong affinity for the FimH adhesin from pathogenic E. coli strains was studied. Mannose-grafted cellulose proved efficient to selectively bind FimH lectin and discriminate pathogenic E. coli strains from non-pathogenic ones. FimH LEctPROFILE plates was used in this study to evaluate the effect of the mannose-grafted cellulose compound over FimH lectin.

Glycobiological interaction studies – a key for the understanding of exopolysaccharide behavior and biofilm formation.

Recently, the group of Parsek have published a study about the function of the exopolysaccharide (PsI) found in Pseudomonas aeruginosa and its interaction with the bacterial lectin LecB that induce stabilisation of the biofilm. This work justifies the interest of glycobiological interactions studies that enable to answer and explain some stayed unknown mecanisms up to now. The expertise of GLYcoDiag in this field is now well known through the GLYcoPROFILE technology platform which is adapted for bacteria-cells glycobiological interactions studies.

Learn more about GLYcoPROFILE

Passos da Silva, D., Matwichuk, M. L., Townsend, D. O., Reichhardt, C., Lamba, D., Wozniak, D. J., Parsek, M. R., Nat. Comm., 2019, 10, 2183 (DOI : 10,1038/s41467-019-10201-4)

Neoglycoclusters

Neoglycoproteins are glycosylated bovine serum albumin (BSA) molecules. In order to improve accessibility and avidity of a carbohydrate-binding proteins, a new version of neoglycoproteins containing spacer arm (i.e. an alkyl spacer or a polyethylene glycol (PEG) chain), were developped and proposed either with monosaccharides/disaccharides or with glycoclusters.

Musa acuminata (BanLec) lectin an original Mannose binding lectin

Musa acuminata lectin (BanLec) is isolated from banana pulp and purified by affinity column chromatography on CarbPROFILE Mannose (see below the focus on GLYcoDiag affinity gel). The  carbohydrate recognition domain of BanLec is mainly specific for mannose. BanLec is available in 2 to 5 mg unlabelled or labelled with biotin or fluorescein.

GLYcoDiag lectin BanLec v1.1