GLYcoDiag offers the implementation of its expertise and technology in adapted forms:
from contracts services, research contracts to development projects and final test product developed for your spcific needs and intended to be used in your facility environment.
Contracts services to suit your molecule sample and your project.
You provide us with your target molecule and we will perform a glycan structure analysis. Using a panel of lectins for screening purposes, we will be able to select the relevant lectins necessary for the study of your molecule. Various options are aiming at a finely tuned interaction profile with lectins to identify the main structural characteristics of the studied glycoforms.
The study will then follow two stages:
A complete analysis of all O-Glycans and N-Glycans can be performed by mass spectrometry if you require it. The sequencing will give you the complete monosaccharide composition (neutrals and sialylated) as well as identifying the glycosylation occupation sites on the glycoprotein.
We offer a full range of structural glycoanalysis services and studies for your molecule using state-of-the-art equipment. Some of them are performed with the aim of a specific partnering with Proteodynamics.
Whole glycoproteins analysis : MADI-TOF.
Glycosylation occupation sites :
Protease digestion followed by glycopeptides mapping by MALDI-TOF or ESI MS.
Glycans structures :
- Fragmentation of glycopeptides (ESI MS/MS, MALDI-PSD).
- N-glycans analysis: N-glycans release (N glycanase), MS analysis.
- O-glycans analysis: Alkaline degradation, MS analysis.
- Neutral monosaccharides composition: Acid hydrolysis, HPAEC-PAD analysis.
- Sialic acid composition: Enzymatic release or mild acid hydrolysis, HPAEC-PAD analysis.
Research contracts :
Our specialisation in glycobiology was applied in projects which cover various fields of applications. The association of our lectin glycoprofiling approach with the structural analysis methods constitute a very powerfull technology platform of glycoanalysis. Depending of the domain of application, various objectives of the research contracts could be followed: custom tests design, glyco-biomarkers identification or glycomimetic drug discovery.
Product projects to provide you with a customised kit.
Our strategy: from study to specific kit product.
If you wish, for example, to control batches of a newly produced recombinant protein, we can develop for you a complete kit that can be used directly in your own laboratory. Microplates with covalently linked lectins will be prepared according to your needs with all the reagents and buffers necessary. This method is quick and efficient, affording a result in just a few hours.
As described in the above figure, the project is made up of three major steps – feasibility study – validation – development and technology transfer. Its purpose is to develop a glycosylation analysis kit specific to your target glycoprotein. A modification in the glycoform population of the analysed protein will be shown in the test as a significant profile variation compared with the reference profile (feasibility study and validation). Finally, development must insure the reproducibility of the kit manufacturing.
Characteristics of the customised kit:
Fast and easy: 4 to 8 samples can be analysed in less than 2 hours.